pYPQ230
Growth in Bacteria
  1. Bacterial Resistance(s): Spectinomycin
  2. Growth Temperature: 37°C
  3. Growth Strain(s): DH5alpha
  4. Copy number: High Copy
Backbone
  1. Vector backbone: pYPQ167
  2. Vector type: Plant Expression, CRISPR ; Gateway compatible LbCpf1 entry clone
Purpose
LbCpf1 Gateway entry plasmid
Cloning Information
  1. Cloning method Restriction Enzyme
  2. 5′ cloning site NcoI-HindIII, (unknown if destroyed)
  3. 3′ cloning site NheI-AatII (unknown if destroyed)
  4. 5′ sequencing primer gtaaaacgacggccag
Gene/Insert
  1. Gene/Insert name: LbCpf1
  2. Species: Lachnospiraceae bacterium ND2006
  3. Tag / Fusion Protein
  4. 5' and 3' NLS
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