pYPQ230
Growth in Bacteria
- Bacterial Resistance(s): Spectinomycin
- Growth Temperature: 37°C
- Growth Strain(s): DH5alpha
- Copy number: High Copy
Backbone
- Vector backbone: pYPQ167
- Vector type: Plant Expression, CRISPR ; Gateway compatible LbCpf1 entry clone
Purpose
LbCpf1 Gateway entry plasmid
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI-HindIII, (unknown if destroyed)
- 3′ cloning site NheI-AatII (unknown if destroyed)
- 5′ sequencing primer gtaaaacgacggccag
Gene/Insert
- Gene/Insert name: LbCpf1
- Species: Lachnospiraceae bacterium ND2006
- Tag / Fusion Protein
- 5' and 3' NLS
