pX333
Growth in Bacteria
  1. Bacterial Resistance(s): Ampicillin
  2. Growth Temperature: 37°C
  3. Growth Strain(s): DH5alpha
  4. Copy number: Unknown
Backbone
  1. Vector backbone: pX333
  2. Backbone size (bp) 9000
  3. Vector type: Mammalian Expression
  4. Promoter CBh; U6
  5. Tag / Fusion Protein
  6. 3XFLAG-Cas9 (N terminal on backbone)
Purpose
(Empty Backbone) Vector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter
Cloning Information
  1. Cloning method Restriction Enzyme
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