pCas9
Growth in Bacteria
- Bacterial Resistance(s): Chloramphenicol
- Growth Temperature: 37°C
- Growth Strain(s): DH5alpha
- Growth instructions :pCas9 is resistant to chloramphenicol (25ug/ml).
- Copy number: Low Copy
Backbone
- Vector backbone: pACYC184
- Backbone size w/o insert (bp) 4244
- Total vector size (bp) 9326
- Vector type: Bacterial Expression, CRISPR ; E.coli
Purpose
Bacterial expression of Cas9 nuclease, tracrRNA and crRNA guide
