pCas9
Growth in Bacteria
  1. Bacterial Resistance(s): Chloramphenicol
  2. Growth Temperature: 37°C
  3. Growth Strain(s): DH5alpha
  4. Growth instructions :pCas9 is resistant to chloramphenicol (25ug/ml).
  5. Copy number: Low Copy
Backbone
  1. Vector backbone: pACYC184
  2. Backbone size w/o insert (bp) 4244
  3. Total vector size (bp) 9326
  4. Vector type: Bacterial Expression, CRISPR ; E.coli
Purpose
Bacterial expression of Cas9 nuclease, tracrRNA and crRNA guide
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