pCAMBIA1304
Description

pCAMBIA1304 contains kanamycin resistance gene for bacterial selection, hygromycin B resistance gene for plant selection and the mgfp5:gusA fusion reporter genes.The pCambia vector backbone is derived from the pPZP vectors.

pCambia1304 vector offers:
1.high copy number in E.coli for high DNA yield 
2.pVS1 replicon for high stability in Agrobacterium
3.small size 
4.restriction sites for modular plasmid modifications and small but adequate polylinkers for introducing DNA of interest
5.bacterial selection with kanamycin 
6.plant selection with hygromycin B 7.simple means to construct translational fusions to mgfp5-gusA reporter genes

Information

Promoter: CaMV 35S promoter
Replicator: pVS1 oriV, ori
Terminator: NOS
Plasmid size: 12361bp
Prokaryotic resistance: Kan
Screening markers: HygR
Cloned strain: DH5 alpha
Culture conditions: 37 centigrade, aerobic, LB
Expressing the host: plant
5'sequencing primers: M13F:TGTAAAACGACGGCCAGT
Use: Plant expression

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